Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data. human oral metagenome (447426)

We developed an open-source R package, decontam, to identify and remove contaminating DNA sequences from marker-gene and metagenomics datasets. We validated decontam on a 16S rRNA gene oral mucosa microbiome dataset generated in our laboratory, and on additional datasets derived from previous studies.