Identification of mitochondrial RNAs in brown adipose tissue

Mitochondria were isolated from brown adipose tissue and the remaining tissue fraction was collected to identify RNA enriched in mitochondria. Mitochondria were isolated from brown adipose tissue, processed by lysis and differential centifugation to collect RNA enriched in the respective compartments.从棕色脂肪组织（brown adipose tissue）中分离线粒体（Mitochondria），并收集剩余的组织组分以鉴定在线粒体中富集的RNA。从棕色脂肪组织中分离线粒体，经裂解与差速离心处理后，收集各亚细胞区室中富集的RNA。