COMPOSITION FOR CLEAVING A TARGET DNA COMPRISING A GUIDE RNA SPECIFIC FOR THE TARGET DNA AND CAS PROTEIN-ENCODING NUCLEIC ACID OR CAS PROTEIN， AND USE THEREOF
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
下载链接：
https://www.forestdata.cn/dataDetail.html?id=709be812-11f7-43ab-87c0-422b707c3ca0
下载链接
链接失效反馈官方服务：
资源简介：
The invention provides a method of introducing double-stranded breaks into a target DNA in a mammalian cell， the method comprising contacting the target DNA with a type II CRISPR/Cas9 system comprising (a) Cas9 protein with one nuclear localization signal added to the C-terminus of the Cas9 protein; and (b) a single-chain guide RNA comprising a crRNA portion fused to a tracrRNA portion， wherein the method is not for treatment of the human or animal body by surgery or therapy， and wherein the method does not modify the germline genetic identity of human beings. The invention further provides a mammalian cell comprising said type II CRISPR/Cas9 system and a method of preparing said mammalian cell.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
