Deep-sequencing of degraded or cleaved RNAs from barley shoot and root growing in low-Pi condition.

RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. PARE libraries were constructed for both barley organs, followed by sequencing of NGS libraries. Overall design: Total RNA isolation, PARE libraries preparation, single-reads (50 cycles) sequencing, Illumina technology, HiSeq 2500, sequencing performed at Fasteris SA (Switzerland).