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Massively parallel reporter assay for programmed ribosomal frameshifting on designed sequence libraries

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP250291
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We designed an oligonucleotide libraries containing a potential frameshifting site, cloned the library variants into a dedicated reporter construct, transfected and integrated these constructs in the genome of K562 cells, and performed FACSseq (as well as targeted RNA sequencing) to determine if and to what extent frameshifting occurs. Overall design: Massively parallel reporter assay for programmed ribosomal frameshifting
创建时间:
2020-06-30
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