Molecular responses of sponge larvae exposed to partially weathered condensate oil
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This data collection consists of sponge larval settlement data, differential gene expression data and microbial amplicon sequence variant (ASV) data associated with an experiment examining the effects of a water accommodated fraction (WAF) of partially weathered condensate oil on the common Indo-Pacific sponge, Phyllospongia foliascens. Sponge larvae were exposed to six WAF treatment dilutions ranging from 0 to 100% for a period of 24-h and processed for Tag-based RNA-Seq and 16S rRNA amplicon sequencing.
Chemical characterisation of the WAF used in this study was perfomed in Negri et al. 2021, with details in the associated repository (https://apps.aims.gov.au/metadata/view/615e208d-f6c7-4b2f-b99e-d4fc50614e48).
For full methodological and analytical details please refer to the full research publication, Luter et al. (in review) its supplementary materials and the associated GitHub repository (https://github.com/open-AIMS/sponge-larvae-condensate-toxicity). Additionally, raw sequencing data can be found in the NCBI Sequence Read Archives under BioProject number PRJNA1012550.
本数据集涵盖与一项实验相关的海绵幼虫附着数据、差异基因表达数据及微生物扩增子序列变异(ASV)数据,该实验探究了部分风化凝析油的水可溶组分(WAF)对常见印太海绵——叶海绵(*Phyllospongia foliascens*)的影响。研究中将海绵幼虫置于6个梯度(0%至100%)的WAF处理液中暴露24小时,随后开展基于标签的RNA测序(Tag-based RNA-Seq)及16S rRNA扩增子测序。
本研究所用WAF的化学表征工作已由Negri等人于2021年完成,详细信息见关联元数据仓储(https://apps.aims.gov.au/metadata/view/615e208d-f6c7-4b2f-b99e-d4fc50614e48)。
若需获取完整的实验方法与分析细节,请参阅Luter等人(已投稿待刊)的研究论文、其补充材料及关联GitHub仓库(https://github.com/open-AIMS/sponge-larvae-condensate-toxicity)。此外,原始测序数据可在NCBI序列读取档案(Sequence Read Archives, SRA)中通过BioProject编号PRJNA1012550获取。
提供机构:
Australian Ocean Data Network



