Analysis of Ppif-/- hearts

To identify differences in gene expression between peptidylprolyl isomerase F (cyclophilin D; Ppif)-null hearts and WT control hearts. RNA was isolated from mice 8 weeks of age to discover gene changes prior to myocardial dysfunction. Three replicates each of Ppif-/- and WT mice.本研究旨在鉴定肽基脯氨酰异构酶F（peptidylprolyl isomerase F，亲环蛋白D；Ppif）敲除小鼠心脏与野生型（Wild Type，WT）对照心脏之间的基因表达差异。为探明心肌功能障碍发生前的基因表达变化，研究人员从8周龄小鼠体内提取RNA。本实验中，Ppif敲除型与野生型小鼠样本各设置3次生物学重复。