Ovarian transcriptional profiles of four mouse strains

The transcriptional profiles of ovarian tissue from four laboratory mouse-inbred strains were obtained with NIA15K-cDNA microarrays and then correlated with the divergent spontaneous ovarian tumor rates and reproductive parameters reported for each strain. Four test samples and one reference sample - common reference design- dye swap - 5 to 6 replicates per sample - test samples pooled from 4-6 animals , 23 arrays采用NIA15K-cDNA微阵列（NIA15K-cDNA microarray）技术，获取了4个实验性近交系小鼠卵巢组织的转录组谱；随后将该转录组谱与各品系已报道的差异性自发卵巢肿瘤发生率及生殖参数进行关联分析。本次实验设置4份测试样本与1份参照样本，采用通用参照设计（common reference design）并开展染料互换（dye swap）；每份样本设置5至6次实验重复，测试样本由4至6只小鼠的组织混合制备，共计使用23张微阵列芯片。