Knapp Hammond et al Human CD34+ Cord Blood

To investigate the molecular diversity of human CD34+ umbilical cord blood, to compare phenotypic and functionally defined subsets, and to identify molecular parameters which may be involved in the regulation of cell functional behaviour. Conclusion: Classical phenotypic subsets contain mixtures of cell types as defined by both molecular characteristics and function. Functional populations within the CD34 fraction show a generally continuous distribution in molecular space rather than showing completely discrete characteristics. 4EQ beads were included in run to allow intensity normalization. All samples were collected, fixed, and stored until time of run at which point all were palladium barcoded to allow staining in a single tube, and thus equalizing antibody exposure across samples.