The role of adaptor protein PINCH mediated signaling pathway in morphogenesis of vasculature [CUT&Tag]

To uncover direct targets of PINCH that could account for its role in vasculature, we performed cut tag analysis on wild type aortic smooth muscle using PINCH1 antibody at E18.5. Our analyses uncovered 50789 significant binding peaks for PINCH, mainly located at intergenic or intronic regions. Overall design: CUT&Tag analyses were performed using the aortic smooth muscle tissue of ctrl mice at E18.5. Tissue samples were dissected under fluorescence microscope.