The relationship between pollen monodiets and the activities of proteolytic system in the fat body and hemolymph of honey bees workers

In this study, the effect of monofloral pollen diets (hazel, rape, pine, buckwheat, phacelia, goldenrod) on the activity of the proteolytic system in the fat body from tergite 3, tergite 5, sternite, and hemolymph of honeybees was investigated. We showed that pollen from rapeseed, phacelia, buckwheat, and goldenrod plants increases the activities of acidic, neutral, and alkaline proteases and their inhibitors in the fat body and hemolymph compared to the group fed with sugar candy only. The activities of proteases and their inhibitors fed with pollen from hazel and pine plants are usually higher compared to the activity of bees fed with sugar candy only, but lower than workers fed sugar candy with entomophilous plants. Moreover, when comparing proteolytic system activity between localizations/segments, the highest activities were observed in tergite 5, regardless of bee age and whether they were fed candy with added pollen.