Gut bacteria induce IgA expression in pituitary hormone-secreting cells during aging

Using the single-cell sequencing technology, the authors aim to make a construction of a single-cell cell atlas of young and old pituitary cells. Moreover, the sorted Ai6+ cells from pituitary of Igha-Cre; Ai6 were also analyzed by single-cell sequencing. The sorted live young and old mouse pituitary cells, as well as sorted Igha-Cre; Ai6+ pituitary cells were captured and analyzed using the 10x Chromium platform.本研究团队借助单细胞测序技术，旨在构建年轻与衰老小鼠垂体细胞的单细胞图谱。此外，研究人员还对Igha-Cre; Ai6小鼠垂体中分选获得的Ai6+细胞开展了单细胞测序分析。所有分选得到的存活年轻及衰老小鼠垂体细胞，以及Igha-Cre; Ai6+垂体细胞，均通过10x Chromium平台完成捕获与测序分析。