ASAP CRN Postmortem-Derived Brain Sequencing Collection

The Postmortem-derived Brain Sequencing Collection is a harmonized repository of scRNAseq sequencing data contributed by two ASAP CRN teams (Team Lee and Team Hafler). These samples were derived from the middle frontal gyrus, hippocampus, substantial nigra, and pre-frontal cortex regions of healthy controls, Parkinson’s Disease, and Alzheimer’s Disease brains. The samples have been harmonized across 10x sequenced data aligned into count tables with cell ranger. The current collection represents the minimum viable product and will be expanded and improved as additional data is uploaded into the ASAP CRN Cloud. When complete, the collection will provide data generated from 1,800+ samples using proteomics, transcriptomics, and sequencing (single-nucleus RNAseq, single-cell RNAseq, bulk RNA-seq, ATAC-seq, long read WGS, and single-nucleus multiome sequencing (paired snRNAseq, snATACseq)) techniques. The analysis workflow for this MVP dataset is available at https://github.com/ASAP-CRN/harmonized-wf-dev