Human spermatozoa as a model for detecting missing proteins in the context of the chromosome-centric Human Proteome Project.

Data from ProteomeXchange, PXD ID: PXD002367. File: Mitchell_40_F03_exclu02.mzml. Published as part of J Proteome Res. 2015 . From the Abstract: {{i}} The chromosome-centric Human Proteome Project (C-HPP) aims at cataloguing the proteins as gene products encoded by the human genome, in a chromosome-centric manner. The existence of products of about 82% of the genes has been confirmed at the protein level. However, the number of so-called missing proteins remains significant. It was recently suggested that the expression of proteins that have been systematically missed might be restricted to particular organs or cell types, for example the testis. Testicular function, and spermatogenesis in particular, is conditioned by the successive activation and/or repression of thousands of genes and proteins, including numerous germ cell- and testis-specific products. Both the testis and post-meiotic germ cells are thus promising sites at which to search for missing proteins and ejaculated spermatozoa are a potential source of proteins whose expression is restricted to the germ cell lineage. A trans-chromosome-based data analysis was performed to catalog missing proteins in total protein extracts from isolated human spermatozoa ... {{/i}}