cDNA clones with reconfirmed activity in hE72-Luc assay^[a] and specific enhancing effects on exon skipping of the hE72-Luc construct in HEK cells, together with their effects on splicing of endogenous mouse <i>Dystrophin</i> transcripts in mdx cells, as determined by qPCR.

[a] cDNA clones classed as reconfirmed hits when activity was >2 fold above hE72-Luc alone in an independent experiment to the initial screen; [b] Assay was performed in the presence of 0.5 µM AON and data represents median fold change for Luciferase assays or average fold change for qPCR; [c] Assay was performed in the absence of AON and data represents median fold change for Luciferase assays or average fold change in the normalized skip/full-length ratio for qPCR; [d] mdx-H2K cells were co-transfected with 0.2 µM AON and cDNA clones prior to qPCR analysis and data represents average fold change in the normalized skip/full-length ratio; [e] mdx-HEK cells were transfected with cDNA clones alone and data represents average fold change in the normalized skip/full-length ratio.