Single-nucleus RNA sequencing of mouse brown adipose tissue

Here, we performed single nucleus RNA sequencing on mouse brown adipose tissues to investigate the gene expression patterns relative to BAT thermogenesis mechanism using denervation model. Single nuclear from mouse brown adipose tissues were isolated and single-nucleus transcriptome libraries were generated本研究对小鼠棕色脂肪组织（mouse brown adipose tissue）开展单细胞核RNA测序（single nucleus RNA sequencing），以借助去神经支配模型（denervation model）探究与棕色脂肪组织（brown adipose tissue, BAT）产热机制相关的基因表达模式。实验过程中从该组织中分离得到单细胞核，并构建了单细胞核转录组文库（single-nucleus transcriptome library）。