Heterodimeric ABC exporter TmrAB (EQ mutant) in ATP-bound outward-facing occluded conformation in the absence of Mg2+

Heterodimeric ABC exporter TmrAB (EQ mutant) in ATP-bound outward-facing occluded conformation in the absence of Mg2+ Descriptor: ADENOSINE-5'-TRIPHOSPHATE, Multidrug resistance ABC transporter ATP-binding and permease protein, Nanobody Nb9F10 Authors: Susac, L, Nocker, C, Tampe, R. Deposit date: 2025-07-15 Release date: 2026-04-08 Last modified: 2026-04-15 Method: ELECTRON MICROSCOPY (3.02 Å) Cite: Single-molecule dynamics reveal ATP binding alone powers substrate translocation by an ABC transporter. Nat Commun, 17, 2026缺乏镁离子（Mg²+）条件下，处于三磷酸腺苷（ATP）结合的朝外闭合构象的异二聚体ABC外排转运蛋白TmrAB（EQ突变体） 描述项：三磷酸腺苷（ATP）、多药耐药ABC转运蛋白ATP结合与通透酶蛋白、纳米抗体Nb9F10 作者：Susac, L、Nocker, C、Tampe, R 提交日期：2025-07-15 发布日期：2026-04-08 最后修改日期：2026-04-15 实验方法：电子显微镜（分辨率3.02埃） 引用文献：单分子动力学揭示仅ATP结合即可驱动ABC转运蛋白完成底物转位。Nat Commun, 17, 2026