Megalobrama amblycephala isolate:blunt snout bream Raw sequence reads

Equal parts from each of the six individual tissue samples per group (brain tissue from HSD-treatment, brain tissue from control, liver tissue from HSD-treatment, liver tissue from control, intestine tissue from HSD-treatment, intestine tissue from control) were pooled and prepared for sequencing. Total RNA was extracted from each tissue using TRIZOL (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. RNA samples were digested by DNase I to eliminate potential genomic DNA. Integrity and size distribution were checked on a Bioanalyzer 2100 with RNA 6000 Nano Labchips (Agilent technologies, Santa Clara, CA, USA). For each pool, one miRNA library were constructed. Totally six miRNA sequencing libraries were constructed and sequenced on an Illumina HiSeq 2500 (Illumina, San Diego, USA) with 50 bp single-end.