SNPs markers of Trypoxylus dichotomus developed by using specific‐locus amplified fragment sequencing (SLAF‐seq) techniques

The Japanese rhinoceros beetle Trypoxylus dichotomus is one of the largest
beetle species in the world and is commonly used in traditional chinese
medicine. Ten subspecies of T. dichotomus and a related Trypoxylus species
(T. kanamorii) have been described throughout Asia, but their taxonomic
delimitations remain problematic. To clarify issues such as taxonomy, and
the degree of genetic differentiation of Trypoxylus populations, we
investigated the genetic structure, genetic variability and phylogeography
of 53 specimens of Trypoxylus species from 44 locations in five Asian
countries (China, Japan, Korea, Thailand, and Myanmar). Using
specific‐locus amplified fragment sequencing (SLAF‐seq) techniques, we
developed 330,799 SLAFs over 114.16M reads, in turn yielding 46,939
high-resolution single nucleotide polymorphisms (SNPs) for genotyping.