Gene expression analysis of Pseudomonas aeruginosa strain CF18 and P. aeruginosa CF18 gacA mutant. Gene expression analysis of Pseudomonas aeruginosa strain CF18 and P. aeruginosa CF18 gacA mutant

We compared gene expression in wild-type P. aeruginosa strain CF18 relative to P. aeruginosa CF18 gacA mutant. Overall design: P. aeruginosa CF18 and P. aeruginosa CF18 gacA mutant were seeded on slow-killing plates. The plates were incubated for 24 h at 37ºC and then for 24 h at 25ºC. °C. Then, bacteria were collected from the plates and total RNAs were extracted (Three biological replicates for WT CF18 and 2 biological replicates for gacA mutant).