Transcriptome analysis of small intestine and liver samples of control and LCWE-induced mice

Purpose:Mucosal immune regulation is considered a key aspect of IgAN immunopathogenesis. Direct experimental evidence clarifying the role of intestinal mucosa attributes in IgAN is lacking. Methods:Small intestine and liver mRNA profiles in control and LCWE-induced mice were generated by deep sequencing. 2 µg total RNAs were used for stranded RNA sequencing library preparation using KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina® . The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Illumina Novaseq 6000. Results:The transcriptional signature showed that numerous genes were highly upregulated in the small intestines of the LCWE-induced mice compared with those in PBS mice. GO analysis of the small intestine revealed an over-representation of genes involved in the Fc gamma R-mediated phagocytosis, B cell receptor signaling pathway, NF-?B signaling pathway, and intestinal immune network for IgA production. The GO analysis of live samples revealed that genes involved in metabolic pathways, galactose metabolism, drug metabolism-other enzymes, and bile secretion were downregulated Conclusions:The intestinal barrier was dysfunctional in the LCWE-induced mice and liver's ability to clear IgA may be reduced. Overall design: Small intestine and liver mRNA profiles of control and LCWE-induce mice