SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in May 2021

Flow cytometry data was collected in May 2021, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs. Since July 2019, sampling has also been conducted at "Gulf" sites: Upper Spencer Gulf (USG) and Gulf St Vincent (GSV), with the original sites identified as "Shelf" sites.

本数据集的流式细胞术（Flow Cytometry）数据采集于2021年5月，采样海域为澳大利亚南部近海。本研究的总体目标是为澳大利亚南部大陆架海域的生态系统建立基础背景资料，阐明上升流/盐度流出事件对微生物群落的影响，最终构建该区域的生物地球化学模型。采样工作依托“恩格林号”科考船（RV Ngerin）开展，作为南澳大利亚综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目的组成部分。每次科考航次中，研究人员均对叶绿素荧光最大值层（Deep Chlorophyll Maximum, DCM）的物理、化学与生物学特性展开调查。本次采集的流式细胞术数据涵盖超微型浮游植物、细菌及病毒三类样本。本研究共计布设6个主要采样站点：其中5个站点位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）与SAM5CB/B5（35.008°S，135.198°E）；另有1个离岸站点B1（36.188°S，136.178°E），位于袋鼠岛西南海域。结合站点间距（14至25海里）、陆架中层海流的平均流速（0.01 m·s⁻¹）与科考船平均航速（9节），可确认各站点采集的水体分属不同水团。研究团队还会在部分时段采集额外样本：位于袋鼠岛的国家参考站（National Reference Station, NRS，35.832°S，136.447°E），以及发生盐度流出事件的南澳大利亚斯宾塞海湾口锚地（SA Spencer Gulf Mouth Mooring, SAM8SG，35.25°S，136.690°E）。自2019年7月起，研究团队还在“海湾”类站点开展采样工作，包括上斯宾塞海湾（Upper Spencer Gulf, USG）与圣文森特湾（Gulf St Vincent, GSV），而最初划定的采样站点则被归类为“陆架”类站点。