Transcriptome of soleus muscle of Tenascin-C deficient 129/SV mice

1 year-old male Tenascin-C deficient 129/SV mice with the targeted insertion of a beta-lactamase cassette in the NcoI site of exon 2 of the TNC gene (Forsberg, E., Hirsch, E., Frohlich, L., Meyer, M., Ekblom, P., Aszodi, A., Werner, S. & Fassler, R. (1996) Proc. Natl. Acad. Sci U. S. A 93, 6594-6599) were used for the study. Animals were derived from the original strain and back-crossed with wildtype 129/SV mice (Institut fur Labortierkunde, University of Zurich). Animals were acclimatized to housing in single cages for one week. Soleus muscle was harvested, total RNA isolated and subjected to transcript profiling with custom microarrays with custom-designed ATLASTM cDNA nylon filter as described (Dapp, C., Schmutz, S., Hoppeler, H. & Fluck, M. (2004) Physiol Genomics 20, 97-107). Keywords: Transcriptome Seven biological replicas本研究使用1岁龄雄性腱生蛋白-C（Tenascin-C）缺陷型129/SV小鼠，该品系小鼠的TNC基因第2外显子的NcoI酶切位点处，靶向插入了β-内酰胺酶盒（Forsberg, E.、Hirsch, E.、Frohlich, L.、Meyer, M.、Ekblom, P.、Aszodi, A.、Werner, S. 与 Fassler, R.，1996年，《美国国家科学院院刊》，93卷，6594-6599页）。实验动物源自原始品系，并与野生型129/SV小鼠回交（苏黎世大学实验动物学研究所）。将实验动物单笼饲养并适应环境1周后，收获其比目鱼肌，提取总RNA，使用搭载定制设计的ATLA™ cDNA尼龙滤膜的定制微阵列开展转录谱分析，实验流程参照已发表方法（Dapp, C.、Schmutz, S.、Hoppeler, H. 与 Fluck, M.，2004年，《生理学基因组学》20卷，97-107页）。关键词：转录组（Transcriptome），7个生物学重复