Structure of an E333Q variant of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex alpha-Glc-1,3-(1,2-anhydro-carba-mannose)
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Structure of an E333Q variant of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex alpha-Glc-1,3-(1,2-anhydro-carba-mannose) Descriptor: (1~{S},2~{S},3~{R},4~{R},6~{R})-4-(hydroxymethyl)-7-oxabicyclo[4.1.0]heptane-2,3-diol, ACETATE ION, Glycosyl hydrolase family 71, ... Authors: Sobala, L.F, Speciale, G, Hakki, Z, Fernandes, P.Z, Raich, L, Rojas-Cervellera, V, Bennet, A, Thompson, A.J, Bernardo-Seisdedos, G, Millet, O, Zhu, S, Lu, D, Sollogoub, M, Rovira, C, Jimenez-Barbero, J, Davies, G.J, Williams, S.J. Deposit date: 2018-03-06 Release date: 2019-09-18 Last modified: 2024-01-17 Method: X-RAY DIFFRACTION (1.12 Å) Cite: An Epoxide Intermediate in Glycosidase Catalysis. Acs Cent.Sci., 6, 2020
解木聚糖拟杆菌（Bacteroides xylanisolvens）来源的GH99家族内切α-甘露聚糖酶（GH99 endo-alpha-mannanase）E333Q突变体与α-Glc-1,3-(1,2-脱水碳环甘露糖（alpha-Glc-1,3-(1,2-anhydro-carba-mannose)）形成复合物的晶体结构。
配体描述：(1S,2S,3R,4R,6R)-4-(羟甲基)-7-氧杂双环[4.1.0]庚烷-2,3-二醇、乙酸根离子（ACETATE ION）、糖苷水解酶家族71（Glycosyl hydrolase family 71）等
作者：Sobala, L.F.、Speciale, G.、Hakki, Z.、Fernandes, P.Z.、Raich, L.、Rojas-Cervellera, V.、Bennet, A.、Thompson, A.J.、Bernardo-Seisdedos, G.、Millet, O.、Zhu, S.、Lu, D.、Sollogoub, M.、Rovira, C.、Jimenez-Barbero, J.、Davies, G.J.、Williams, S.J.
存档日期：2018-03-06
发布日期：2019-09-18
最后修改日期：2024-01-17
实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率1.12埃（Å）
引用文献：《糖苷酶催化中的环氧中间体》（An Epoxide Intermediate in Glycosidase Catalysis），发表于ACS Central Science，2020年，第6卷
创建时间：
2018-03-06
