RNASeq analysis of in vitro generated myeloid-derived supressor cells (MDSCs)

Several mechanisms have been implicated in MDSC-mediated suppression of immune responses. To better understand the mechanism(s)/pathway(s) MDSCs utilize to suppress immune responses, we performed a transcriptome profiling of CD11b+CD11c+ (good suppressors) and CD11b+CD11c- (poor suppressors) MDSCs. Comparison of the respective transcriptomes allowed us to identify target genes associated with immunosuppression. Overall design: Here we analyse in vitro generated myeloid-derived supressor cells (MDSCs) separated into two populations – CD11b+CD11c+ and CD11b+CD11c- with six replicates each for a total of 12 RNA-Seq samples.