Transcriptome of Zebrafish Intestine under Fe'i and Cavendish Banana Diet

Fe'i banana (Musa troglodytarum) fruit is carotenoid-rich and has been proposed to be utilized as a functional food. We report the first transcriptome of zebrafish (Danio rerio) intestine that were subjected to 8 weeks of Fe'i banana and Cavendish (Musa acuminata) diet. We found that Fe'i consumption in zebrafish modulated genes related to innate immunity. Adult healthy zebrafish were divided into 3 diet groups: commercial feed (CO), Cavendish (CV), and Fe'i banana (FE). Zebrafish in commercial feed diet group was used as control and fed Tetramin (Tetra, Germany). Ripe Cavendish and Fe'i fruit pulp were mixed with commercial feed into 50% banana-containing granule by lyophilization. Treatment lasted for 8 weeks. At day 56, total RNA of intestine from each diet group were sequenced. Transcriptome assembly were done using Tophat-Cufflink pipeline.