Normalized library of protein domains (Domainome) produced with InteractomeSeq approach. RNAbinding Domains RIDOME

Nowadays the advent of High-Throughput Sequencing technologies is transforming multiple research fields as well as the analysis of the intrinsic heterogeneity of phage libraries. Phage display technology coupled with deep sequencing (Interactome-sequencing) was introduced in 2010 and holds the potential to circumvent the traditional laborious picking and testing of individual phage rescued clones. However, from a bioinformatic point of view the analysis of this kind of data was always performed by adapting tools designed for other purposes, thus not taking into account the noise background typical of Interactome-sequencing approach and the heterogeneity of the data. InteractomeSeq is a web server allowing domainome data analysis from either Eukaryotic and Prokaryotic genomic phage libraries generated and selected by following an Interactome-sequencing approach. InteractomeSeq allows users to upload raw-sequencing data and to obtain an accurate characterization of domainome/epitome profiles after setting the parameters required to tune the analysis. The release of this tool is relevant for the scientific and clinical community, because InteractomeSeq will fill an existing gap in the field of large-scale biomarkers profiling, reverse vaccinology, and structural/functional studies, thus contributing essential information for gene annotation or antigen identification. InteractomeSeq is freely available at: https://InteractomeSeq.ba.itb.cnr.it