16S rRNA Amplicon Sequences of Anopheles gambiae Midgut Samples

Glyphosate (GLYPH) is an herbicide that is commonly used in agriculture and is the active ingredient in Roundup. Studies have shown that ingestion of GLYPH by honeybees increases the susceptibility of the insects to natural infection, a phenomenon attributed to perturbation of the resident gut microbiome. One of the aims of this study was to determine if GLYPH has similar effects on the microbiome of Anopheles gambiae mosquitoes. Mosquitoes were drugged with varying doses of GLYPH, and their gut microbiota were analyzed by 16S rRNA amplicon sequencing. Results indicate that while GLYPH does not alter the bacterial load of the A. gambiae gut microbiome, the makeup of the community is altered in a dose-independent manner.草甘膦（Glyphosate，缩写GLYPH）是一种农业生产中广泛使用的除草剂，亦是农达（Roundup）的活性成分。现有研究表明，蜜蜂摄入GLYPH后，其对自然病原感染的易感性会显著提升，该现象被认为源于宿主肠道常驻微生物群的稳态扰动。本研究的核心目标之一，便是探究GLYPH是否会对冈比亚按蚊（Anopheles gambiae）的肠道微生物群产生类似影响。研究人员以不同剂量的GLYPH处理按蚊，随后通过16S rRNA扩增子测序技术对其肠道微生物群展开分析。结果显示，尽管GLYPH并未改变冈比亚按蚊肠道微生物群的细菌载量，但该微生物群落的组成结构却以剂量非依赖性的方式发生了改变。