Retroviral vector adsorption efficiency using the RBV-LTS method.

MT-MFR3 vector was preloaded into RN-coated 24-well plates, which were then subjected to the RBV-static, -Spin, or -LTS methods. Scaled-up preloading was also performed using an RN-coated PL325 bag with the RBV-LTS method. After the preloading, the residual retroviral RNAs were directly extracted using extraction solvent. The amount of retroviral RNA present was quantified using the qPCR method. Adsorption efficiencies were calculated by dividing the number of recovered RNA copies by the total number of input RNA copies. Experiments were conducted in triplicate, and all data represent mean ± SD. Statistical analysis was performed by Student t-test between active adsorptions (Spin or LTS) and passive adsorption (Static). (*p<0.05, **p<0.01, ***p<0.001).