MitoFI development dataset: 500 blinded confocal fields of mitochondria with three-observer morphology labels

Development data for MitoFI. 500 maximum-intensity projections of the mitochondrial channel (MitoTracker Red) from fixed Huh7 cells and mouse embryonic fibroblasts, acquired on an Olympus FV3000 confocal microscope across 36 imaging sessions (lateral pixel size 0.046–0.196 µm). Fields carry random identifiers; no file links an identifier to an experimental condition. Three observers rated every field blind as elongated, intermediate ("normal"), fragmented or uncertain (labels_*.csv; two-of-three majority in labels_consensus.csv); per-field pixel size, Z depth and imaging session are in covariates.csv. MitoFI_scribbles_ilastik.zip contains the ilastik pixel-classification project with the mitochondria and background scribbles used to train the released pixel classifier, together with the 31 fields it was drawn on (Olympus, Leica and Allen Institute images). Column definitions are in DATA_DICTIONARY.md.