PhIP-Seq/VirScan Coronavirus phage display assay in maternal-infant dyads

We investigated antibody linear epitope binding and transplacental transfer using the PhIP-seq/VirScan SARS-CoV-2 Spike protein phage display array in mother-infant dyads at the time of birth. SARS-CoV-2 antibody linear epitope binding after vaccination during pregnancy showed high levels of binding in carboxy terminal of N-terminal, S1/S2 cleavage site, and S2 — with multiple immunodominant regions found in the majority of mothers and infants.本研究针对分娩时刻的母婴对，采用PhIP-seq/VirScan 新型冠状病毒（SARS-CoV-2）刺突蛋白（Spike protein）噬菌体展示阵列，探究了抗体线性表位结合活性与胎盘转运情况。孕期接种疫苗后诱导产生的SARS-CoV-2抗体，其线性表位结合活性在氨基末端区域的羧基末端、S1/S2切割位点以及S2区域呈现高水平结合特征；多数母婴样本中均可检测到多处免疫优势区域。