Native Australian seedlings exhibit novel strategies to acclimate to repeated heatwave events

Heatwaves are becoming more intense and frequent. Plant photosystem thermal thresholds can vary with species, but also shift in response to environmental triggers. Both upper and lower thresholds can acclimate to repeated heatwaves through ecological stress memory, where prior exposure primes them for subsequent events. The extent to which acclimation to repeated heat stress events varies among environmental origin and/or species is unknown. Different acclimation strategies might reflect biome of origin, or may be species-specific.
For 12 species from two contrasting biomes – extreme desert and benign coastal temperate – we investigated responses to two simulated heatwaves, via shifts in upper and lower critical temperatures of photosystem II, and the difference between these thresholds, thermal tolerance breadth (TTB). 
Biome of origin had no effect on thermal tolerance. Observed differences among species following heat events suggested two possible acclimatory strategies. In some..., , ## Native Australian seedlings exhibit novel strategies to acclimate to repeated heatwave events

Contains 15 files:

(1) Analysis - R scripts required to replicate analysis and figures in the manuscript and supplementary material:
DRYAD biome and species lmer models.R
DRYAD Delta models.R
DRYAD Figure 2.R
DRYAD supp.R

(2) Datasets (11 files total):

dfcold.csv
dfhot.csv
dfTTB.csv

For analysis of effect of treatment and time period on seedling tcrit_hot, tcrit_cold and thermal tolerance breadth

Columns:

* stage - the time period of when the measurement was taken; 1,2,3,4
* biome - desert (extreme), coastal temperate (benign)
* species - Acacia longifolia, Acacia salicina, Acacia victoriae, Banksia integrifolia, Capparis mitchellii, Carex appressa, Casuarina pauper, Eucalyptus largiflorens, Flindersia maculosa, Lomandra longifolia, Melaleuca hypericifolia, Pittosporum undulatum
* treatment - 0_HW, control; 1_HW, one heatwave; 2_HW, two heatwaves
* block_rep - replicate; 1, 2, 3, 4, 5...,