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Circadian regulation of lung repair and regeneration

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NIAID Data Ecosystem2026-05-01 收录
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To elucidate the mechanistic basis of the regenerative deficiency seen with clock disruption we undertook an unbiased approach with single-cell RNA sequencing (scRNA-seq) of epithelial and mesenchymal cells from Bmal1-/-, Bmal1creERT2/+, and their respective WT littermates Lungs from embryonic Bmal1-/-, Bmal1+/+ controls, Bmal1creERT2/+, and Bmal1creERT2neg littermates were dissected, and single-cell preparation was obtained as described above. Single cell suspension was sorted as DARQ7-CD31-CD45- lung cells

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2023-12-18
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