Peptidase deletion strains generated in this study.

Gene names were determined where possible by following the recommended naming guidelines for C. neoformans [49]. NatR is nourseothricin resistance. An asterisk indicates the observation of a phenotype in subsequent mutant characterization studies (S8 and S9 Figs). Evidence for activity in YNB or DMEM conditioned media was determined in subsequent experiments analyzing proteolytic activity in media conditioned by the peptidase deletion strains (Figs 2 and 3, S4 and S5 Figs). Proteins identified in the present study’s secretome proteomics are indicated.