Retrotransposon Ty1 integration targets specifically positioned asymmetric nucleosomal DNA segments in tRNA hotspots

Using a TIP-seq protocol (specifically isolating transposon insertion junctions) we determined that the Ty1 retrotransposon targets tRNA genes and, in particular, we determined that the transposon inserts into nucleosomal DNA in an asymmetric pattern.本研究采用TIP-seq技术（特异性分离转座子插入连接位点的实验方案），证实Ty1反转录转座子（Ty1 retrotransposon）的靶向整合位点为转运RNA（tRNA）基因；尤为关键的是，本研究进一步揭示该转座子以不对称模式插入核小体DNA（nucleosomal DNA）中。