Raw data Western blot

Cytokeratin 80 (KRT80) is a protein type belonging to the keratin family, that has been correlated with more aggressive tumor phenotypes, higher rates of proliferation, enhanced migratory and invasive capabilities, and poorer clinical outcomes in different cancers. Although KRT80 may act as a mediator of oncogenic signaling, the molecular mechanisms in CRC development still need to be evaluated. Here, we aimed to investigate the miR-195-5p molecular mechanism related to the modulation of KRT80, underlying its role in CRC and emphasizing the potential properties of this miRNA for therapy. In summary, this study provides evidence that KRT80 is significantly overexpressed in CRC patients. miR-195-5p effectively restores normal KRT80 expression in CRC cell lines and AOM/DSS-treated mice, highlighting its regulatory role. Additionally, the critical oncogenic properties of KRT80 were demonstrated, that once silenced, suppressed the tumor growth. These findings further reinforce the clinical potential of miR-195-5p in affecting the keratin expression and influencing CRC progression.