eDNA samples from the gut content and gut lining of Lanternfish for DNA metabarcoding using Nanopore short-read sequencing method along the 110E meridian Second International Indian Ocean Expedition (IIOE-2) expedition RV Investigator voyage IN2019_V03 (2019)

Lanternfish samples were collected during the 2nd International Indian Ocean Expedition (https://iioe-2.incois.gov.in/), on board a 94m oceanographic research vessel (RV Investigator, CSIRO, Hobart, Tasmania), which took place between 13 May 2019 and 14 June 2019. This study uses the gut (content and lining) from juvenile lanternfish (Hygophum) specimens to compare the short-read sequencing capability of the portable Nanopore MinION with the Illumina MiSeq. Primers common in dietary DNA metabarcoding work (COI Leray primers and 18S rRNA V4 Zhan primers) were used, with an additional comparison of cost-effective COI Lobo primers (targeting the same COI fragment) for the proficiency in species detection of a broad range of taxa. Supplimentary data available at https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fedn3.374&file=edn3374-sup-0001-supinfo.xlsx. All data analyses was done using R v4.1.0 script available at https://auckland.figshare.com/articles/onlineresource/Short-readDNAmetabarcodingusing_Nanopore/21482826 Voyage details (metadata, projects, other datasets either online or as downloads, publications and reports, events, maps etc) can be accessed at https://www.marine.csiro.au/data/trawler/surveydetails.cfm?survey=IN2019V03 If this data has been used in any products, please acknowledge with the following: We acknowledge the use of the CSIRO Marine National Facility (https://ror.org/01mae9353) in undertaking this research.

本研究的灯笼鱼样本采集自第二届印度洋国际考察航次（https://iioe-2.incois.gov.in/），科考船为长94米的海洋学考察船“调查者号”（RV Investigator），隶属于塔斯马尼亚州霍巴特的澳大利亚联邦科学与工业研究组织（CSIRO），航次执行时间为2019年5月13日至2019年6月14日。本研究以幼体灯笼鱼（灯笼鱼属*Hygophum*）样本的肠道（内含物与肠道内壁）为研究材料，对比便携式纳米孔测序（Nanopore）MinION平台与Illumina MiSeq平台的短读长测序性能。实验采用了饮食DNA宏条形码研究中常用的引物对（COI基因Leray引物与18S rRNA基因V4区Zhan引物），同时额外设置了成本效益更优的COI基因Lobo引物（靶向同一段COI基因片段），以对比不同引物对广谱类群的物种检测效能。补充数据集可通过以下链接获取：https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fedn3.374&file=edn3374-sup-0001-supinfo.xlsx。所有数据分析均基于R v4.1.0版本脚本完成，相关脚本可通过以下链接获取：https://auckland.figshare.com/articles/onlineresource/Short-readDNAmetabarcodingusing_Nanopore/21482826。本航次的详细信息（包括元数据、项目信息、在线/可下载的其他数据集、已发表文献与报告、航次事件、地图等）可通过以下链接查询：https://www.marine.csiro.au/data/trawler/surveydetails.cfm?survey=IN2019V03。若基于本数据集产出相关成果，请按照以下方式致谢：本研究使用了澳大利亚联邦科学与工业研究组织海洋国家设施（https://ror.org/01mae9353）提供的科考资源。