GFP-H3.3 and -H3.1 are mobilized during HSV-1 infection.

(A) Digital fluorescent micrographs of Vero cells expressing GFP-H3.1 (H3.1) or -H3.3 (H3.3) at 4 h after infection with 30 PFU/cell of strain KOS (KOS) or mock-infection (Mock). Images were taken before (0) or 1 s after photobleaching. (B) Mobilization of GFP-H3.3 or -H3.1 was examined from 4 to 5 (4 hpi) or 7 to 8 (7 hpi) hpi by FRAP; error bars, standard errors of the means (SEM). (C) Normalized levels of free GFP-H3.3 or -H3.1 relative to mock-infected cells at 4 or 7 hpi, respectively; error bars, SEM; dashed line, normalized levels in mock-infected cells. (D) Frequency distribution plots of the percentage of free GFP-H3.3 or -H3.1 in individual cells at 4 or 7 hpi; dotted line, one standard deviation (SD) above the average level of free GFP-H3.3 or -H3.1 in mock-infected cells. (E) Average initial rates of normalized fluorescence recovery relative to mock-infected cells at 4 hpi; error bars, SEM. (F) Frequency distribution plots of the initial rate of normalized fluorescence recovery of GFP-H3.3 or -H3.1 per individual cell; dotted line, one SD above the average initial rate of GFP-H3.3 or -H3.1 normalized fluorescence recovery in mock-infected cells. **, P<0.01; *, P<0.05; n.s., not significant.