Data From: Laisk measurements in the non-steady-state: tests in plants exposed to warming and variable CO2 concentrations

Light respiration (RL) is an important component of plant carbon balance and a key parameter in photosynthesis models. RL is often measured using the Laisk method, a gas exchange technique that is traditionally employed under steady-state conditions. However, a non-steady-state dynamic assimilation technique (DAT) may allow for more rapid Laisk measurements. In two studies, we examined the efficacy of DAT for estimating RL and the parameter Ci* (the intercellular CO2 concentration where rubisco’s oxygenation velocity is twice its carboxylation velocity), which is also derived from the Laisk technique. In the first study, we compared DAT and steady-state RL and Ci* estimates in paper birch (Betula papyrifera) growing under control and elevated temperature and CO2 concentrations. In the second, we compared DAT-estimated RL and Ci* in hybrid poplar (Populus nigra L. x P. maximowiczii A. Henry ‘NM6’) exposed to high or low CO2 concentration pre-treatments. The DAT and steady-state methods p..., These data come from two experiments in which foliar gas exchange via the Laisk method was used to collect estimates of light respiration and Ci* (the intercellular CO2 concentration where rubisco's oxygenation velocity is twice its carboxylation velocity). All gas exchange data were collected using the LI-6800.  The Laisk method involves collecting photosynthetic CO2 response curves at multiple light intensities. Traditionally the Laisk method has been employed under steady-state conditions, but more recently a non-steady-state technique, called the dynamic assimilation technique (DAT), has become available in gas exchange systems such as the LI-6800. 
In experiment 1, Laisk curves were collected using both the steady-state and DAT methods on paper birch grown under six treatments reflecting possible climate change scenarios for boreal forests.
In experiment 2, Laisk curves were collected with DAT. Light respiration was also measured using the Yin method. In this method, a photosynthet..., The datafile is a .csv file that can be opened in most programs and/or software including Microsoft Excel, R and Python.