Chemical-shift perturbation of Tim10-mutants F33Q and V29K
收藏doi.org2025-03-23 收录
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http://doi.org/10.17632/c9bp4dt5bz.1
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NMR analysis of two Tim10-mutants of TIM9·10.
(A-D) F33QTim10. (E-H) V29KTim10. The BEST-TROSY 1H-15N NMR spectra (A,E) of the mutants (blue) are similar to those of wild-type TIM9·10 (black). (B, C, F, G) Residue-wise chemical-shift perturbations, calculated as the weighted sum of 1H and 15N chemical shifts.
(D, H) Localization of residues with significant chemical-shift perturbations are shown in green and light blue, and the mutated residues, F33 and V29, are shown as dark blue sticks. Overall, chemical-shift perturbations are modest and located in the vicinity of the mutated residue, revealing that the mutants are structurally similar to the wild-type TIM9·10 complex.
对TIM9·10复合物中的两种Tim10突变体进行的核磁共振分析。图(A-D)展示的是F33QTim10突变体，图(E-H)展示的是V29KTim10突变体。突变体的最佳TROSY 1H-15N核磁共振光谱(A,E)与野生型TIM9·10(黑色)相似。图(B, C, F, G)展示了按残基划分的化学位移扰动，计算方法为1H和15N化学位移的加权总和。图(D, H)展示了具有显著化学位移扰动的残基定位，以绿色和浅蓝色表示，突变残基F33和V29以深蓝色棍状表示。总体而言，化学位移扰动幅度适中，位于突变残基附近，表明这些突变体在结构上与野生型TIM9·10复合物相似。
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