Intracellular quantitative distribution of GdTbF3-based nanohybrids for X-ray photodynamic therapy: achieving statistical significance with

We have developed promising "all-in-one" GdF3-based nanohybrids for both imaging and X-ray photodynamic therapy (X-PDT) on Spectral Photon Counting Scanner CT (SPCCT). We obtained promising results on their intracellular quantitative distribution but on a very limited number of cells per condition. Indeed, XRF nanoimaging is a low-throughput technique. We are developing an AI-based guided-XRF system to reach mandatory statistically significant sampling. In the current proposal, we will benefit from these developments by performing intelligent on-line analysis and decision-making, reducing the involvement of an operator, and increasing the throughput. We expect that the results of this proposal will contribute to the fast development of imaging/therapy in X-ray SPCCT and also allow us to showcase the in-house developments to speed up future nanoimaging experiments.