Effect of Pak4 deficiency on transcriptome in liver of mice

PAK4 has emerged as a promising target for anti-cancer drug development. However, its role in oxidative stress conditions remains elusive. We investigated the effects of PAK4 signaling on hepatic ischemia/reperfusion (I/R) injury. To elucidate molecular mechanisms, we performed RNA-sequencing analysis using littermate WT and hepatocyte-specific Pak4 KO mice in I/R condition. Transcriptomic analysis enabled us to find the main signaling nodes altered by Pak4 deficiency in close association with antioxidant response. Examination of RNA profile in liver of WT and hepatocyte-specific Pak4 KO mice.PAK4已成为抗肿瘤药物开发的极具潜力的靶点。然而，其在氧化应激状态下的作用仍尚不明确。本研究探究了PAK4信号通路对肝缺血再灌注（I/R）损伤的影响。为阐明其分子机制，本研究在I/R模型条件下，利用同窝野生型（WT）及肝细胞特异性Pak4敲除（KO）小鼠开展了RNA测序分析。通过转录组分析，我们鉴定出Pak4缺失后发生显著改变的核心信号节点，且这些节点与抗氧化应答通路紧密关联。本研究同时对野生型及肝细胞特异性Pak4敲除小鼠的肝脏RNA表达谱进行了检测。