Single cell sequencing of WT and hb-egfaKO zebrafish spinal cord, at 1 week after sham or transection injury.

We identified hb-egfa as a secreted factor necessary and sufficient for spinal cord regeneration in zebrafish. To elucidate the Hb-egfa mechanism of action, we perforemd single cell sequencing of WT and hb-egfaKO zebrafish spinal cord. Adult zebrafish were subjected to spinal cord transection. 2 mm rostral and caudal to the transection site were collected at 1 week post injury. Spinal cord of sham-injured animals was collected as control. Samples were and subjected to the 10X Genomics pipeline for single cell sequencing (4 samples in total, WT 1 wpi, WT sham, hb-egfaKO 1 wpi, hb-egfaKO sham).