Modification of fat mass through targeting distal enhancer of Ptrf

We revealed a close positive relationship between Ptrf transcription and adipose mass in mice, and single-nucleus transcriptomics indicated the involment of impariment of adipogenic differentiation, adipocyte metabolism, transportation of multiple nutrients and altered regulatory network in Ptrf knockout-induced decrease of fat mass. We analyzed inguinal white adipose from 6-month-old WT and PTRF KO mice using single-nucleus sequencing.