anterior pituitary transcriptomics following a high fat diet: impact of oxidative stress on cell metabolism

Anterior pituitary cells are highly active with regards to protein synthesis and secretion, processes which depend heavily on mitochondrial ATP production and functional endoplasmic reticula. It is well known that obesity adds an allostatic overload to tissues, requiring them to adapt to inflammation and oxidative stress. Therefore, we hypothesized that the pituitary is highly vulnerable to the stress of high fat diet-induced weight gain. In this study, we utilized a 10-15 week high fat diet (HFD, 60%) plus a thermoneutral housing paradigm, testing both male and female FVB.129P mice. We quantified serum hormones and cytokines, characterized the metabolic phenotype, and defined changes in the pituitary transcriptome using single-cell RNA-seq. Weight gain was significant by 3 weeks in HFD mice, and by 10 weeks all HFD groups had gained 20 g. HFD females (15 weeks) had increased energy expenditure and decreased activity. All HFD groups showed increases in serum leptin, Il-6, resistin, MCP-..., , , # Data from: anterior pituitary transcriptomics following a high fat diet: impact of oxidative stress on organelle pathology Anterior pituitary cells are highly active with regards to protein synthesis and secretion, processes which depend heavily on mitochondrial ATP production and functional endoplasmic reticula. It is well known that obesity adds an allostatic overload to tissues, requiring them to adapt to inflammation and oxidative stress. Therefore, we hypothesized that the pituitary is highly vulnerable to the stress of high fat diet-induced weight gain. In this study, we utilized a 10-15 week high fat diet (HFD, 60%) plus a thermoneutral housing paradigm, testing both male and female FVB.129P mice. We quantified serum hormones and cytokines, characterized the metabolic phenotype, and defined changes in the pituitary transcriptome using single-cell RNA-seq. Weight gain was significant by 3 weeks in HFD mice, and by 10 weeks all HFD groups had gained 20 g. HFD females (15 week...垂体前叶细胞（Anterior pituitary cells）在蛋白质合成与分泌过程中活性极高，而这两类生理过程高度依赖线粒体三磷酸腺苷（ATP）生成与功能性内质网的正常运作。众所周知，肥胖会给机体组织带来稳态超载（allostatic overload），迫使组织适应炎症与氧化应激状态。据此，我们提出假说：垂体极易受到高脂饮食诱导的体重增加所引发的应激损伤。本研究采用10~15周龄高脂饮食（HFD，60%脂肪供能比）结合热中性饲养范式，对雄性与雌性FVB.129P小鼠开展实验。我们定量检测了血清激素与细胞因子水平，表征了小鼠的代谢表型，并通过单细胞RNA测序（single-cell RNA-seq）分析了垂体转录组的变化。高脂饮食小鼠在造模3周时体重即出现显著增长，至10周时所有高脂饮食组小鼠体重均增加了20g。高脂饮食喂养的雌性小鼠（造模15周时）能量消耗升高、活动量降低。所有高脂饮食组小鼠的血清瘦素、白介素-6（Il-6）、抵抗素、单核细胞趋化蛋白（MCP-...）水平均有所升高。# 数据来源：高脂饮食后垂体转录组学研究：氧化应激对细胞器病理的影响 垂体前叶细胞在蛋白质合成与分泌过程中活性极高，此类过程高度依赖线粒体ATP生成与功能性内质网的正常运作。众所周知，肥胖会给机体组织带来稳态超载，迫使组织适应炎症与氧化应激状态。据此，我们提出假说：垂体极易受到高脂饮食诱导的体重增加所引发的应激损伤。本研究采用10~15周龄高脂饮食（HFD，60%脂肪供能比）结合热中性饲养范式，对雄性与雌性FVB.129P小鼠开展实验。我们定量检测了血清激素与细胞因子水平，表征了小鼠的代谢表型，并通过单细胞RNA测序分析了垂体转录组的变化。高脂饮食小鼠在造模3周时体重即出现显著增长，至10周时所有高脂饮食组小鼠体重均增加了20g。高脂饮食喂养的雌性小鼠（造模15周时……）