Mapping data for HTS assembled genomes

There was no significant difference in the diversity of viruses infecting pumpkins in the different sub-regions in Uganda. To undertake this study, High Throughput sequencing was used to determine the diversity of viruses infecting pumpkin. To carry out this study, sixty-five symptomatic and 10 asymptomatic samples were collected and organized into five groups of 15 leaf samples were preserved in RNAlater® solution at -20°C while in transit to the Agricultural Research Council (ARC) in the Republic of South Africa for HTS. Raw reads from the Illumina HiSeq sequencing platform were assembled using the Genome Detective 1.132 bioinformatics pipeline (https://www.genomedetective.com/app/typingtool/virus/; May 20, 2024). Furthermore, the genome isolates were mapped on existing genomes in the GenBank to determine the identity levels.乌干达不同亚区域内侵染南瓜的病毒多样性无显著差异。本研究采用高通量测序（High Throughput Sequencing, HTS）技术，明确侵染南瓜的病毒多样性。研究期间共采集65份显症南瓜样本与10份无症状南瓜样本，将其划分为5组，每组含15份叶片样本。样本在转运至南非共和国农业研究委员会（Agricultural Research Council, ARC）开展高通量测序期间，保存于RNAlater®保存液中并置于-20℃环境。采用Illumina HiSeq测序平台产出的原始读段，通过Genome Detective 1.132生物信息学分析流程（https://www.genomedetective.com/app/typingtool/virus/；2024年5月20日）完成组装。此外，将基因组分离株与GenBank数据库中已收录的参考基因组进行比对，以确定序列同源性水平。