Essential role of Helicobacter pylori apolipoprotein N-acyltransferase (Lnt) in stomach colonization

Deleting lnt changes the number of acyl chains on lipoproteins and the number of acyl chains on lipoproteins impacts the innate immune response through TLR2 signaling. We treated primary human gastric epithelial cells with a purified lipoprotein from wild-type or lnt mutant H. pylori and performed RNAseq. Differential gene expression analysis indicated lipoprotein from the lnt mutant induced a more robust TLR2 response. Deidentified primary human gastric epithelial cells were left untreated or treated with purified triacylated Lpp20-HA, diacylated Lpp20-HA, Pam3-CSK4, or Pam2-CSK4 for 4 hours