NanoString Analysis of Gene Expression in B16.F10 Tumors Treated with the cGAS Activator, NanoISD. NanoString Analysis of Gene Expression in B16.F10 Tumors Treated with the cGAS Activator, NanoISD

Characterization of the immune profile (i.e. tumor RNA) of a murine B16.F10 tumor 6 hours post a single 100 µL intratumoral injection of 2 µg NanoISD, Free D-PDB at an equivalent dose, or PBS. Overall design: 12 biological samples are analyzed, comprising 3 treatment groups (i.e. PBS, D-PDB, and NanoISD), each with 4 biological replicates. PBS and D-PDB represent the negative control and vehicle control, respectively. Each biologial sample was evaluated for the mRNA expression of 43 myeloid-related genes along with 5 house-keeping genes (i.e. Gapdh, Gusb, Hdac3, Rpl19, and Tbp)