Expression analysis of DamP mRNAs using expressed molecular barcodes

We have generated 979 yeast strains in which the natural 3' UTR of essential gene mRNAs has been replaced by the same long 1.4 kb artificial 3' UTR (DAmP modification)我们成功构建了979株酵母菌株，在这些菌株中，必需基因信使RNA（messenger RNA, mRNA）的天然3'非翻译区（3' Untranslated Region, 3' UTR）均被替换为统一的1.4 kb长人工3'非翻译区（3' UTR），该修饰方式为DAmP修饰