Centrosome clustering was enhanced in p53-/- MEF cells with extra centrosomes after RhoA or ROCK inhibition.

p53-/- MEF cells were treated with Cyto-B (0.5 µg/ml) for 12 h, then released and treated with 0.5 µg/ml C3 transferase (RhoA inhibitor) or 0.5 µM H1152 (ROCK inhibitor) for 8h, and stained with antibodies against γ-tubulin and α-tubulin for centrosomes and microtubules, respectively. Nuclei were stained with DAPI. Note that only cells with more than 3 centrosomes at metaphase were analyzed. The percentage of cells with indicated mechanism was shown separately. Mean ± s.d. from three independent experiments.*p2 test, compared with control cells.